MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03950cam a22004694a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
15373675 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
IN-BhIIT |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20170304140848.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
080717s2009 nyua b 001 0 eng |
| 010 ## - LIBRARY OF CONGRESS CONTROL NUMBER |
|---|
| LC control number |
2008031690 |
| 016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER |
|---|
| Record control number |
101479171 |
| Source |
DNLM |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9780879698683 (hardcover : alk. paper) |
|
|---|
| International Standard Book Number |
9780879698676 (pbk. : alk. paper) |
| 035 ## - SYSTEM CONTROL NUMBER |
|---|
| System control number |
(OCoLC)235945971 |
|
|---|
| System control number |
(OCoLC)ocn235945971 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
DNLM/DLC |
| Transcribing agency |
DLC |
| Modifying agency |
NLM |
| -- |
YDXCP |
| -- |
BTCTA |
| -- |
C#P |
| -- |
BWX |
| -- |
CDX |
| -- |
COO |
| -- |
DLC |
| 042 ## - AUTHENTICATION CODE |
|---|
| Authentication code |
pcc |
| 050 00 - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
QP551 |
| Item number |
.B265 2009 |
| 060 00 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
|---|
| Classification number |
2008 M-826 |
|
|---|
| Classification number |
QU 25 |
| Item number |
B3115 2009 |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
572/.6078 |
| Edition number |
22 |
| Item number |
SIM/B |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Basic methods in protein purification and analysis : |
| Remainder of title |
a laboratory manual / |
| Statement of responsibility, etc. |
edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Cold Spring Harbor, N.Y. : |
| Name of publisher, distributor, etc. |
Cold Spring Harbor Laboratory Press, |
| Date of publication, distribution, etc. |
c2009. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
vii, 436 p. : |
| Other physical details |
ill. ; |
| Dimensions |
29 cm. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc |
Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks. |
|
|---|
| Formatted contents note |
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Proteins |
| General subdivision |
Purification |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
1744 |
|
|---|
| Topical term or geographic name as entry element |
Proteins |
| General subdivision |
Analysis |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
1745 |
|
|---|
| Topical term or geographic name as entry element |
Proteins |
| General subdivision |
isolation & purification |
| Form subdivision |
Laboratory Manuals. |
| 9 (RLIN) |
1746 |
|
|---|
| Topical term or geographic name as entry element |
Chromatography |
| General subdivision |
methods |
| Form subdivision |
Laboratory Manuals. |
| 9 (RLIN) |
1747 |
|
|---|
| Topical term or geographic name as entry element |
Electrophoresis |
| General subdivision |
methods |
| Form subdivision |
Laboratory Manuals. |
| 9 (RLIN) |
1748 |
|
|---|
| Topical term or geographic name as entry element |
Immunoprecipitation |
| General subdivision |
methods |
| Form subdivision |
Laboratory Manuals. |
| 9 (RLIN) |
1749 |
|
|---|
| Topical term or geographic name as entry element |
Proteins |
| General subdivision |
analysis |
| Form subdivision |
Laboratory Manuals. |
| 9 (RLIN) |
1750 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Simpson, Richard J. |
| 9 (RLIN) |
1751 |
|
|---|
| Personal name |
Adams, Peter D. |
| Fuller form of name |
(Peter David) |
| 9 (RLIN) |
1752 |
|
|---|
| Personal name |
Golemis, Erica. |
| 9 (RLIN) |
1753 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Materials specified |
Table of contents only |
| Uniform Resource Identifier |
<a href="http://www.loc.gov/catdir/toc/fy0903/2008031690.html">http://www.loc.gov/catdir/toc/fy0903/2008031690.html</a> |
| 906 ## - LOCAL DATA ELEMENT F, LDF (RLIN) |
|---|
| a |
7 |
| b |
cbc |
| c |
orignew |
| d |
1 |
| e |
ecip |
| f |
20 |
| g |
y-gencatlg |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Technical Reference Book |
