Basic methods in protein purification and analysis :

Basic methods in protein purification and analysis : a laboratory manual / edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis. - Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2009. - vii, 436 p. : ill. ; 29 cm.

Includes bibliographical references and index.

Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks. Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.

9780879698683 (hardcover : alk. paper) 9780879698676 (pbk. : alk. paper)

2008031690

101479171 DNLM


Proteins--Purification--Laboratory manuals.
Proteins--Analysis--Laboratory manuals.
Proteins--isolation & purification--Laboratory Manuals.
Chromatography--methods--Laboratory Manuals.
Electrophoresis--methods--Laboratory Manuals.
Immunoprecipitation--methods--Laboratory Manuals.
Proteins--analysis--Laboratory Manuals.

QP551 / .B265 2009

572/.6078 / SIM/B

2008 M-826 QU 25 / B3115 2009

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