000			03950cam a22004694a 4500
001			15373675
003			IN-BhIIT
005			20170304140848.0
008			080717s2009 nyua b 001 0 eng
010			_a 2008031690
016	7		_a101479171 _2DNLM
020			_a9780879698683 (hardcover : alk. paper)
020			_a9780879698676 (pbk. : alk. paper)
035			_a(OCoLC)235945971
035			_a(OCoLC)ocn235945971
040			_aDNLM/DLC _cDLC _dNLM _dYDXCP _dBTCTA _dC#P _dBWX _dCDX _dCOO _dDLC
042			_apcc
050	0	0	_aQP551 _b.B265 2009
060	0	0	_a2008 M-826
060	1	0	_aQU 25 _bB3115 2009
082	0	0	_a572/.6078 _222 _bSIM/B
245	0	0	_aBasic methods in protein purification and analysis : _ba laboratory manual / _cedited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis.
260			_aCold Spring Harbor, N.Y. : _bCold Spring Harbor Laboratory Press, _cc2009.
300			_avii, 436 p. : _bill. ; _c29 cm.
504			_aIncludes bibliographical references and index.
505	0		_aIntroduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks.
505	0		_aIntroduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.
650		0	_aProteins _xPurification _vLaboratory manuals. _91744
650		0	_aProteins _xAnalysis _vLaboratory manuals. _91745
650	1	2	_aProteins _xisolation & purification _vLaboratory Manuals. _91746
650	2	2	_aChromatography _xmethods _vLaboratory Manuals. _91747
650	2	2	_aElectrophoresis _xmethods _vLaboratory Manuals. _91748
650	2	2	_aImmunoprecipitation _xmethods _vLaboratory Manuals. _91749
650	2	2	_aProteins _xanalysis _vLaboratory Manuals. _91750
700	1		_aSimpson, Richard J. _91751
700	1		_aAdams, Peter D. _q(Peter David) _91752
700	1		_aGolemis, Erica. _91753
856	4	1	_3Table of contents only _uhttp://www.loc.gov/catdir/toc/fy0903/2008031690.html
906			_a7 _bcbc _corignew _d1 _eecip _f20 _gy-gencatlg
942			_2ddc _cTRB
999			_c8217 _d8217